Fig 1: Localization of receptors for lysosomal hyrolases, Cathepsin B and insulin at the Golgi and cytoplasm in INS1 832/13 cells. (A–F) INS1 832/13 cells were immunostained for insulin (pan-insulin antibody; green), TGN38 (not shown) and CPE (A), M6PR (C), or IGF2R (E). (A, C, and F) Image segmentation of the TGN38 volume was used to identify Golgi-staining regions. (B, D, and F) Line intensity profiles of the Golgi staining regions for each protein are shown. (G) Single slice from a confocal image obtained from INS1 832/13 cells expressing mCherry and Apex tagged proinsulin (red) and stained with an antibody against Cathepsin B (green). Area outlined in white denotes the Golgi region which was marked using GM130 staining which is not shown here.